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RNA is the dissolved in DEPC water and heated to 60 C for 10 minutes

Arthritis Rheumatol (2012) 64(9):293746

It is well established that -cells are more vulnerable to ROS due to their low antioxidant capacity and poor ability to repair oxidatively damaged DNA [22,31]

25 These studies indicate that extracardiac tissue-derived EVs and their contained cargos may serve as alternative candidates to mediate exercise-associated cardioprotection