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Description

2009;89:1960S1980S

CABI (Centre for Agriculture and Bioscience International)

The results showed a higher concentration of tumor necrosis factor (TNF-) and proinflammatory cytokines, interleukin 6 (IL-6) and interleukin 1 (IL-1), during 72 h incubation with the mitogen

Plates were rinsed in PBS twice and fluorescence was measured at emission 485 nm/excitation 535 nm in end point mode, using a fluorescence microtiter plate reader (VICTOR X3, PerkinElmer, Akron, OH, USA)