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For determining the median lethal dose (LD 50 ), five groups of ten female mice (8-12 weeks old) were challenged by footpad injection with 5 10 7 , 5 10 6 , 5 10 5 , 5 10 4 , or 0 PFU/ml of CHIKV (181/25 strain)

Berlin, New York: De Gruyter (1988)

According to them, the use of acatalasemic cells proved the great importance of the catalase which can be the first in defense of the H 2 O 2 , once the GSH increasing or reduction (which would allow the elevation of the GPx activity) didnt alter the general antioxidant activity in the normal or acatalasmic cells

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