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Both residues are within an ideal hydrogen bond distance from the N-atom of the -amino group of PMP after the formation of the Schiff-base linkage between K274 (K314 of the precursor protein) and PLP (the N-atom of the bound PMP/PLP)

Ann N Y Acad Sci 1031:249262

Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)

Minor allele frequency (MAF) and effect size of the most significant SNPs was negatively correlated with large effect alleles being most rare