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Briefly, 150 L of reaction mixtures containing combinations of 10 mM L-Cys, 1 mM PLP, and 50 M Fe 3+ in the presence of 100 M AzMC probe were placed in 96-well plates and analyzed at various timepoints on SpectraMax i3 multimode plate reader through bottom reading (37 C, Exc/Emi = 350/445 nm, photomultiplier tube (PMT): medium) with 10 min interval

however MET+EGFR inhibition synergistically decreased cell viability, demonstrating the combinations higher effectiveness (69)