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Construction of markerless single and double glycerol kinase deletion mutants saci_1117 , saci_2033 and saci_1117 saci_2033 To obtain the markerless GK deletion mutants of saci_1117 and saci_2033 , the plasmids pSVA12818 and pSVA12822 were constructed

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HIV envelope protein gp120 and the regulatory virus protein, Tat, are involved in BBB breakdown