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In a recent clinical trial, Reverse Transcription Polymerase Chain Reaction (RT-PCR) for single nucleotide polymorphism genotyping and multiplex PCR/capillary electrophoresis for fragment analysis was employed, examining the role of 11 alleles in a 10-reward gene panel, which reflects brain reward circuitry activity in 121 chronic opioid users.167 The cohort consisted of 55 men and 66 women, with average ages of 54 and 53 years, respectively, and included Caucasians, African Americans, Hispanics, and Asians

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