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Supplementary Materials The following supporting information can be downloaded at: Author Contributions Conceptualization, R.F

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However, since GHcy-SO 2 H contains a CS bond rather than an SS bond, it cannot undergo the final hydrolytic step of the catalytic cycle and instead accumulates as a dead-end product, leading to time-dependent inactivation of the enzyme ( -glutamyl transpeptidase ( FIGURE 12 TST TST, also known as rhodanese, is a mitochondrial matrix enzyme that participates in both cyanide detoxification and mitochondrial sulfide oxidation (Way, 1984)