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cerevisiae BY4741 to replace the GSH1 promoter with the strong constitutive promoter pTEF1 by homologous recombination, with pgsh1 D:: HIS3MX6-pTEF1 transformants selected by growth in YNB minus histidine

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For example, Trx redox pairs are generally more reducing than GSH/GSSG, and while Trx1 shows a redox potential of -280 and -300 mV in the cytosol and nucleus, Trx2 shows an, even more, reducing redox pair of -340 and -360 mV in mitochondria (9, 48)