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For lopinavir/ritonavir and for darunavir/cobicistat, a moderate risk of relevant PK interactions (higher exposure of antidepressants metabolised by CYP3A4 and CYP2D6 requiring dose adjustments) and PD (QT prolongation with concomitant administration of lopinavir/ritonavir and several SSRIs, TCAs, mianserin, mirtazapine, or trazodone) interactions could be predicted

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An Aminex HPX-87H ion exchange column (Bio-Rad, United States) was used, as described previously (Varela et al., 2003)